一类带参数的泛函微分方程的正周期解的存在性(英文)

利用锥上不动点理论,本文研究了一类非线性泛函微分方程正周期解的存在多样性和ω-周期解的不存在性.获得了一些新的结果.应用这些新的结果,讨论了一类带参数的血细胞生成模型,给出该模型的正周期解的存在性,此结果利用以前的方法是无法得到的.     

The Insufficiency Intake of Dietary Micronutrients Associated with Malnutrition-Inflammation Score in Hemodialysis Population

The relations between dietary micronutrient, nutritional status and inflammation in hemodialysis patients are still unclear. A cross-sectional study was performed in hemodialysis population. 75 hemodialysis patients from South China participated in the dietary and nutritional assessment. Clinical and dietary data were collected. Nutritional status was assessed by Malnutrition-Inflammation Score (MIS) in addition to related anthropometric measurements. And according to the MIS score, the whole hemodialysis patients were divided into normal nutrition group and malnutrition group. The results showed that mid arm circumference (MAC) negatively correlated with MIS (r = −0.425; P = 0.002). The area under the ROC curve (AUC) for MAC was 0.737 (0.614–0.859). Comparing with the normal nutritional group, lower dietary selenium (Se), copper (Cu), iodine (I) and manganese (Mn) intake were observed among patients with malnutrition (P<0.05). While no significant differences of diverse vitamins were found. In conclusion, MAC was effective indicator for assessing nutritional and inflammatory status (P<0.05). The reduction of dietary Se, Cu, I and Mn intake level may be alarming markers for malnutrition and inflammatory status in hemodialysis patients.     

使用哈迪-温伯格不平衡检验来检测多个标记-疾病关联(英文)

多位点单体型的可用性为疾病易感位点(DSL)的遗传分析提供了有价值的工具.DSL定位可以通过检测患病个体中一个标记位点的哈迪-温伯格不平衡(HWD)来实现.本文拓展了HWD检验到多个标记位点对DSL进行单体型关联分析.我们调查了HWD检验的统计功效并与常用的x~2统计量进行比较.结果表明HWD检验具有高的功效且比常用的x~2统计量的功效高.     

喀斯特地区农村劳动力转移对生态恢复的影响及其区域差异——基于酉阳和沿河的农户调查数据

喀斯特地区的生态恢复对于促进国土空间的生态修复、实现区域生态安全具有重要意义。基于酉阳县和沿河县的383份农户调查数据，从微观的农户尺度运用Tobit模型分析喀斯特地区农村劳动力转移对生态恢复的影响，并关注其对酉阳县和沿河县生态恢复的影响差异。研究结果表明：①研究区劳动力转移现象明显，47.67%的农村劳动力发生了转移；②研究区生态恢复情况总体较好，酉阳县的生态恢复好于沿河县；③农村劳动力转移通过农业劳动力的流失效应和非农收入的替代效应促进了喀斯特地区的生态恢复；④劳动力转移的程度不同，对生态恢复的作用强度也不同。沿河相对滞后的劳动力转移程度，使得其生态恢复稍弱于酉阳。为促进喀斯特地区生态恢复，政府应有序引导农村劳动力向非农就业转移，提高转移农户的非农化程度。     

The Influence of Age and Gender on Skin-Associated Microbial Communities in Urban and Rural Human Populations

Differences in the bacterial community structure associated with 7 skin sites in 71 healthy people over five days showed significant correlations with age, gender, physical skin parameters, and whether participants lived in urban or rural locations in the same city. While body site explained the majority of the variance in bacterial community structure, the composition of the skin-associated bacterial communities were predominantly influenced by whether the participants were living in an urban or rural environment, with a significantly greater relative abundance of Trabulsiella in urban populations. Adults maintained greater overall microbial diversity than adolescents or the elderly, while the intragroup variation among the elderly and rural populations was significantly greater. Skin-associated bacterial community structure and composition could predict whether a sample came from an urban or a rural resident ~5x greater than random.     

外泌体源性miRNAs在肺癌发生发展中的作用

外泌体(exosomes)是细胞分泌的纳米级细胞外囊泡.外泌体通过释放其内的生物活性大分子,比如微小RNA(microRNA,miRNA)到受体细胞,从而介导细胞间交流通讯. MiRNAs作为一类主要在转录后水平负向调控靶mRNAs的非编码RNAs,其在外泌体中含量最为丰富.在肺癌中,miRNAs经肿瘤细胞分泌的外泌体转运释放而发挥重要的作用.本文主要讨论了外泌体源性miRNAs在肺癌发生发展的各个阶段,包括血管生成、细胞增殖、侵袭转移、免疫逃逸、耐药等方面的作用,以及其在作为新型肺癌诊断和预后标志物方面的临床价值.     

肿瘤特异性蛋白B7-H4纳米抗体的筛选和鉴定

羊驼体内存在天然缺少轻链的重链抗体,克隆重链抗体可变区(VHH),即可构建单域抗体(single-domain antibodies,sdAbs),又称纳米抗体(nanobody,Nb)。利用非免疫羊驼噬菌体文库筛选肿瘤特异性蛋白B7-H4的纳米抗体,经过4轮淘选,ELASE鉴定阳性克隆噬菌体,测序获得其DNA序列后体外转录为mRNA,将修饰纯化后的mRNA转染到肿瘤细胞,利用细胞免疫荧光检测mRNA在肿瘤细胞内是否表达,Western印迹进一步验证其表达情况;通过CCK-8法鉴定其对肿瘤细胞的增殖抑制能力;划痕实验鉴定其对肿瘤细胞迁移能力的影响;Transwell法鉴定其对肿瘤细胞的侵袭能力的影响;裸鼠荷瘤模型瘤旁注射mRNA,鉴定其在体内实验对肿瘤组织的作用。结果显示,通过淘选获得1个高亲和性的噬菌体株菌H6;DNA测序并导出的氨基酸序列符合羊驼纳米抗体结构;将其mRNA导入肿瘤细胞,能有效表达出纳米抗体H6;转染H6-mRNA的肿瘤细胞(0.84±0.08)与未转染H6-mRNA的对照组(1.83±0.04)相比,其增殖能力明显受到抑制,P<0.01,其迁移和侵袭能力(78.60±5.36)明显低于空白对照组(197.80±21.04),效果优于B7-H4 mRNA的siRNA(95.40±16.56);在裸鼠乳腺癌模型中能有效抑制肿瘤生长,效果优于紫杉醇和B7-H4 mRNA的siRNA。这说明筛选所得抗B7-H4纳米抗体H6能特异结合B7-H4蛋白并封闭其功能,导致肿瘤细胞的增殖、迁移和侵袭受到抑制。该结果为利用B7-H4作为治疗癌症的靶点提供了实验基础。